4W78

Crystal structure of the ChsH1-ChsH2 complex from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-01-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	51.785, 134.096, 90.420
Unit cell angles	90.00, 91.58, 90.00

Refinement procedure

Resolution	37.600 - 1.541
R-factor	0.1771
Rwork	0.177
R-free	0.20460
RMSD bond length	0.009
RMSD bond angle	1.154
Refinement software	PHENIX ((phenix.refine: dev_1391))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.600
High resolution limit [Å]	1.520
Number of reflections	180565
<I/σ(I)>	19.2
Completeness [%]	99.2
Redundancy	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	24% PEG 3350, 20 mM CaCl2, 20 mM CdCl2, 20 mM CoCl2