4W77

Crystal Structure of Full-Length Split GFP Mutant D21H/K26C Disulfide and Metal-Mediated Dimer, P 21 21 21 Space Group, Form 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	62.120, 68.290, 121.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.790 - 3.100
R-factor	0.2243
Rwork	0.217
R-free	0.29080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.011
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	60.790
High resolution limit [Å]	3.100
Number of reflections	9841
<I/σ(I)>	9.86
Completeness [%]	99.9
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	50% PEG200, 0.2M MgCl2, 0.1M NaCacodylate pH 6.5