4W76
Crystal Structure of Full-Length Split GFP Mutant D21H/K26C Disulfide and Metal-Mediated Dimer, P 21 21 21 Space Group, Form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.160, 66.840, 121.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.500 - 2.350 |
R-factor | 0.238 |
Rwork | 0.233 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.010 |
RMSD bond angle | 1.249 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 60.500 |
High resolution limit [Å] | 2.345 |
Number of reflections | 22306 |
<I/σ(I)> | 10.12 |
Completeness [%] | 99.2 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 3M NaCl, 0.1M Bis-Tris pH 5.5 |