4W74
Crystal Structure of Full-Length Split GFP Mutant E115C/T118H With Metal Mediated Crystal Contacts, P 1 21 1 Space Group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.440, 119.790, 130.570 |
Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
Resolution | 88.270 - 2.100 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
RMSD bond length | 0.013 |
RMSD bond angle | 1.572 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: DEV_1555)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 88.270 |
High resolution limit [Å] | 2.099 |
Number of reflections | 119482 |
<I/σ(I)> | 8.06 |
Completeness [%] | 98.8 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 17% PEG10000, 0.1M Ammpnium acetate, 0.1M Bis-Tris pH 5.5 |