4W70
Periplasmically Produced Monomeric Single Domain Antibody (sdAb) C22A/C99V variant against Staphylococcal enterotoxin B (SEB) at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2013-07-23 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 87.826, 87.826, 47.062 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.910 - 2.280 |
| R-factor | 0.19008 |
| Rwork | 0.188 |
| R-free | 0.23638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tyu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.251 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.910 | 2.380 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.138 | 0.411 |
| Number of reflections | 6169 | |
| <I/σ(I)> | 9.61 | 2.79 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.3 | 3.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 0.1 M HEPES pH 7.0, 1.6 M Ammonium Sulfate |
