4W6W
Co-complex structure of the lectin domain of F18 fimbrial adhesin FedF with inhibitory nanobody NbFedF6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 101.361, 101.361, 62.768 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 87.780 - 2.510 |
| R-factor | 0.19974 |
| Rwork | 0.197 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x1o |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.869 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.800 | 2.640 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Rmerge | 1.029 | |
| Number of reflections | 13039 | |
| <I/σ(I)> | 12.6 | 2.7 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 10.9 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 160 mM NaCl, 80 mM Bis-Tris pH 5.5, 20% PEG-3350 |
