4W6F

Crystal Structure of Full-Length Split GFP Mutant K26C Disulfide Dimer, P 32 2 1 Space Group, Form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 32 2 1
Unit cell lengths	121.980, 121.980, 140.090
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	84.340 - 2.700
R-factor	0.208
Rwork	0.204
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.009
RMSD bond angle	1.303
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	84.340
High resolution limit [Å]	2.700
Number of reflections	33538
<I/σ(I)>	5.64
Completeness [%]	100.0
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	10% 2-propanol, 0.1M Imidazole pH 8.0, 5mM NiCl2