4W6B

Crystal Structure of a Superfolder GFP Mutant K26C Disulfide Dimer, P 21 21 21 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-04-23
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.151, 90.356, 102.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.690 - 1.900
R-factor	0.169
Rwork	0.167
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.015
RMSD bond angle	1.176
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall
Low resolution limit [Å]	44.690
High resolution limit [Å]	1.900
Number of reflections	37298
<I/σ(I)>	7.28
Completeness [%]	98.4
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	14% PEG-4000, 0.2M MgCl2, 0.1M Tris pH 8.5