4W69

Crystal Structure of Full-Length Split GFP Mutant Q157C Disulfide Dimer, P 43 21 2 Space Group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0717
Spacegroup name	P 43 21 2
Unit cell lengths	133.760, 133.760, 88.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.580 - 3.975
R-factor	0.31
Rwork	0.307
R-free	0.33500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.005
RMSD bond angle	0.912
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1555))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.580	4.120
High resolution limit [Å]	3.975	3.975
Rmerge		1.660
Number of reflections	7344
<I/σ(I)>	16.94	2.1
Completeness [%]	99.9	99.3
Redundancy	15	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.4M MgFormate, 0.1M Acetate pH 4.6, 2%w/v benzamidine