4W65

Crystal Structure of Glycosyl hydrolase family protein from Mycobacterium fortuitum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9787
Spacegroup name	P 21 21 21
Unit cell lengths	45.990, 57.840, 89.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.998 - 1.380
R-factor	0.1538
Rwork	0.153
R-free	0.17180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rq0
RMSD bond length	0.009
RMSD bond angle	1.114
Phasing software	BALBES
Refinement software	PHENIX ((PHENIX.REFINE: DEV_1769))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.420
High resolution limit [Å]	1.380	1.380
Rmerge	0.046	0.485
Number of reflections	48609
<I/σ(I)>	26.91	4.3
Completeness [%]	97.7	96
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	EBS INTERNAL TRACKING NUMBER 500 MM NACL, 2 MM DTT, 0.025% SODIUM AZIDE, 5% GLYCEROL, 0.4 LISO4; 20% EG ADDED AS CRYOPROTECTANT, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K