4W5Q
The Crystal Structure of Human Argonaute2 Bound to a Guide and Target RNA Containing Seed Pairing from 2-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.500, 116.655, 70.066 |
| Unit cell angles | 90.00, 92.26, 90.00 |
Refinement procedure
| Resolution | 35.294 - 3.101 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w5o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.218 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.880 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.396 | |
| Number of reflections | 16052 | |
| <I/σ(I)> | 10.6 | 2.5 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | PEG 3350, Isopropanol, Phenol, Tris, Magnesium |
