4W5P
Prp peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 11.593, 17.152, 19.837 |
| Unit cell angles | 90.00, 100.70, 90.00 |
Refinement procedure
| Resolution | 19.492 - 1.151 |
| R-factor | 0.1208 |
| Rwork | 0.119 |
| R-free | 0.15080 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.344 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.190 |
| High resolution limit [Å] | 1.150 | 2.480 | 1.150 |
| Rmerge | 0.119 | 0.100 | 0.147 |
| Total number of observations | 10485 | ||
| Number of reflections | 2529 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 90.5 | 99.7 | 51.5 |
| Redundancy | 4.1 | 6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-tris, 0.1 M ammonium acetate, and 40 % MPD |
