4W5M
Prp peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 47.481, 9.437, 22.621 |
| Unit cell angles | 90.00, 95.18, 90.00 |
Refinement procedure
| Resolution | 23.644 - 1.200 |
| R-factor | 0.1955 |
| Rwork | 0.193 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
| Rmerge | 0.071 | 0.065 | 0.099 |
| Total number of observations | 7028 | ||
| Number of reflections | 2911 | ||
| <I/σ(I)> | 11.1 | ||
| Completeness [%] | 86.9 | 85.2 | 49.2 |
| Redundancy | 2.4 | 2.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M Bis-tris, 2% 2-methyl-2,4-pentanediol (MPD), and 1.1 M ammonium sulfate |
