4W4N
Crystal structure of human Fc at 1.80 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-14 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.710, 79.290, 138.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.188 - 1.800 |
R-factor | 0.20132 |
Rwork | 0.200 |
R-free | 0.24436 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l6x |
RMSD bond length | 0.020 |
RMSD bond angle | 2.125 |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.200 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.787 |
Number of reflections | 51158 | |
<I/σ(I)> | 18.4 | 2.4 |
Completeness [%] | 99.1 | 97.3 |
Redundancy | 6.6 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 0.2M calcium acetate, 0.1M sodium cacodylate, 19% PEG 8000 |