4W4M

Crystal structure of PrgK 19-92

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	170
Detector technology	CCD
Collection date	2013-06-20
Detector	RAYONIX MX300HS
Wavelength(s)	0.9511
Spacegroup name	P 21 21 2
Unit cell lengths	88.120, 112.100, 112.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.390 - 3.200
R-factor	0.24783
Rwork	0.246
R-free	0.27951
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.930
Data reduction software	iMOSFLM
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	79.390
High resolution limit [Å]	3.200
Number of reflections	17949
<I/σ(I)>	4.2
Completeness [%]	100.0
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	80 mM Phosphate buffer pH 4.0, 20 mM Tris pH 7.0, 25 % PEG 300, 20 mM MgCl2, 20 mM NaCl