4W4J

Crystal structure of EspG3 from the ESX-3 type VII secretion system of M. smegmatis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.980,0.957
Spacegroup name	P 32 2 1
Unit cell lengths	95.210, 95.210, 171.540
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.605 - 2.803
R-factor	0.195
Rwork	0.190
R-free	0.23540
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.401
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1727))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge		3.150
Number of reflections	22760
<I/σ(I)>	19.8	1.2
Completeness [%]	100.0	100
Redundancy	21.9	22.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.2 M lithium sulfate, 0.1 M phosphate-citrate pH 4.2, 10% (v/v) isopropanol