4W4H

Escherichia coli tryptophanase in holo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-01-18
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	112.440, 112.440, 232.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.970 - 2.890
R-factor	0.22931
Rwork	0.227
R-free	0.27764
RMSD bond length	0.019
RMSD bond angle	1.814
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.950
High resolution limit [Å]	2.890	2.890
Rmerge	0.167	0.750
Number of reflections	31660
<I/σ(I)>	19	2.9
Completeness [%]	98.7	99.2
Redundancy	9.4	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	293	15% (w/v) ammonium sulfate in 25 mM potassium acetate, pH 5.4, and 3 to 10 mM B-mercaptoethanol