4W2Q
Anti-Marburgvirus Nucleoprotein Single Domain Antibody C Complexed with Nucleoprotein C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-06-01 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.664, 98.461, 68.498 |
| Unit cell angles | 90.00, 96.23, 90.00 |
Refinement procedure
| Resolution | 41.680 - 2.700 |
| R-factor | 0.2252 |
| Rwork | 0.220 |
| R-free | 0.27390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6app |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.410 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rpim | 0.092 | 0.398 |
| Number of reflections | 20770 | 3013 |
| <I/σ(I)> | 8.2 | 1.9 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% polyethylene glycol 6000, 0.2M magnesium chloride, 0.1M 1,2,3-hexanetriol, 0.1 M sodium acetate pH 5 |
