4W2P
Anti-Marburgvirus Nucleoprotein Single Domain Antibody C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.392, 49.506, 65.406 |
| Unit cell angles | 87.74, 84.77, 79.41 |
Refinement procedure
| Resolution | 26.772 - 1.770 |
| R-factor | 0.167 |
| Rwork | 0.165 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6apo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.034 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.650 | 1.860 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rpim | 0.058 | 0.332 |
| Number of reflections | 36665 | 5165 |
| <I/σ(I)> | 8.6 | 2.3 |
| Completeness [%] | 91.6 | 88.5 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% polyethylene glycol 550 monomethyl ether/polyethylene glycol 20000, 0.1M carboxylic acids mix (sodium formate, ammonium acetate, sodium citrate, sodium/potassium tartrate, sodium oxamate), 0.1M imidazole/MES pH 6.5 |
