4W2O
Anti-Marburgvirus Nucleoprotein Single Domain Antibody B Complexed with Nucleoprotein C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-23 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.998, 108.657, 141.272 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.328 - 3.200 |
| R-factor | 0.2325 |
| Rwork | 0.227 |
| R-free | 0.28460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6app |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.564 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rpim | 0.081 | 0.297 |
| Number of reflections | 15378 | 2189 |
| <I/σ(I)> | 10.6 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% polyethylene glycol 4000, 0.16M ammonium sulfate, 20% glycerol, 0.08M sodium acetate pH 4.6 |
