4W1T
Structure of the Ssl1 laccase mutant H99Y with depleted type-2 copper ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97858 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.490, 104.558, 163.408 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.280 - 1.550 |
| R-factor | 0.15048 |
| Rwork | 0.149 |
| R-free | 0.17986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m3h |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.309 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.470 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.228 | |
| Number of reflections | 128732 | |
| <I/σ(I)> | 23.4 | 6.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Hepes pH 7, 1.0-1.3 ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride |
