4V9P
Control of ribosomal subunit rotation by elongation factor G
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 361.140, 360.510, 429.730 |
| Unit cell angles | 90.00, 103.22, 90.00 |
Refinement procedure
| Resolution | 70.000 - 2.900 |
| Rwork | 0.215 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 70.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 1984536 |
| Completeness [%] | 89.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD, KSCN, pH 6.5, microbatch, temperature 291K |
