4V9G
RC-LH1-PufX dimer complex from Rhodobacter sphaeroides
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.077, 415.075, 129.818 |
| Unit cell angles | 90.00, 105.75, 90.00 |
Refinement procedure
| Resolution | 20.390 - 7.780 |
| R-factor | 0.22939 |
| Rwork | 0.228 |
| R-free | 0.25767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MODEL OBTAINED BY FITTING 2D CRYO- ELECTRONMYCROSCOPY IMAGES |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.630 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.390 | 8.400 |
| High resolution limit [Å] | 7.780 | 7.780 |
| Rmerge | 0.093 | 0.905 |
| Number of reflections | 6831 | |
| <I/σ(I)> | 5.6 | 1 |
| Completeness [%] | 82.0 | 80 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | 292 | The core dimer complex was concentrated to an optical density of ~100 at 875 nm and washed in (0.42%) n-nonyl- -D maltopyranoside to exchange the purification detergent, n-dodecyl- -D-maltopyranoside. Crystals grew in 14.00% PEG400, 0.10 M N-cyclohexyl-3-aminopropanesulfonic acid (CAPS) pH 10.5, and 1.0% spermidine., VAPOR DIFFUSION, SITTING DROP, temperature 292K |
