4V99

The Crystallographic Structure of Panicum Mosaic Virus

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-02-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	411.740, 403.900, 412.460
Unit cell angles	90.00, 89.65, 90.00

Refinement procedure

Resolution	50.010 - 2.900
R-factor	0.255
Rwork	0.251
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c8n
RMSD bond length	0.009
RMSD bond angle	1.410
Data reduction software	d*TREK
Data scaling software	d*TREK (9.2L)
Phasing software	CNS (1.1)
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.010	2.810
High resolution limit [Å]	2.710	5.840	2.710
Rmerge	0.136	0.065	0.346
Total number of observations		1234096	39843
Number of reflections	2279719
<I/σ(I)>	6.8	13.3	2.5
Completeness [%]	63.0	99.6	4.9
Redundancy	3.14	3.34	2.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	10% PEG400, 0.1 M citric acid, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K