4V96
The structure of a 1.8 MDa viral genome injection device suggests alternative infection mechanisms
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 163.530, 287.360, 323.140 |
| Unit cell angles | 90.00, 101.73, 90.00 |
Refinement procedure
| Resolution | 35.970 - 3.800 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.26410 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 4.000 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 284699 | |
| <I/σ(I)> | 8.72 | 1.28 |
| Completeness [%] | 99.2 | 99.1 |
| Redundancy | 3.4 | 3.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.08M Trizma pH8.0, 0.1M KCl, 15% PEG2000MME, VAPOR DIFFUSION, temperature 293K |
