4V8J

Crystal structure of the bacterial ribosome ram mutation G347U.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	210.197, 446.163, 620.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.900
R-factor	0.2432
Rwork	0.242
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.203
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.7_641)
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.100
High resolution limit [Å]	3.900	3.900
Rmerge	0.354	1.409
Number of reflections	497544
<I/σ(I)>	5.7	1.8
Completeness [%]	94.3	90.9
Redundancy	6.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	3.5-4.5% PEG 20K, 3.5-4.5% PEG550 MME, 0.1M TRIS-ACETATE, 0.2M KSCN, 10MM MGCL2, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K