4V87

Crystal structure analysis of ribosomal decoding.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-05-11
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	210.190, 451.050, 621.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	153.762 - 3.100
R-factor	0.2008
Rwork	0.201
R-free	0.23990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	0.690
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: dev_810))

Data quality characteristics

	Overall	Inner shell
Low resolution limit [Å]	300.000	300.000
High resolution limit [Å]	3.000	50.000
Rmerge	0.332	0.049
Number of reflections	1165415	279
<I/σ(I)>	12.52	48.52
Completeness [%]	100.0	88.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K