4V86
Structure-function Analysis of Receptor-binding in Adeno-Associated Virus Serotype 6 (AAV-6)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-08 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9186 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 354.793, 363.897, 371.863 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.210 - 3.003 |
R-factor | 0.2513 |
Rwork | 0.251 |
R-free | 0.28620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.203 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.210 |
High resolution limit [Å] | 3.003 |
Rmerge | 0.120 |
Number of reflections | 332221 |
<I/σ(I)> | 3.7 |
Completeness [%] | 42.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 4-6% PEG6000, 100 mM HEPES, 50 mM magnesium chloride,, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |