4V7W

Structure of the Thermus thermophilus ribosome complexed with chloramphenicol.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979490
Spacegroup name	P 21 21 21
Unit cell lengths	210.180, 448.400, 621.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.571 - 3.000
Rwork	0.244
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.316
Number of reflections	1004197
<I/σ(I)>	7.73	1.27
Completeness [%]	98.6	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1