4V7T
Crystal structure of the E. coli ribosome bound to chloramphenicol.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1158 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 211.456, 434.084, 621.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.146 - 3.194 |
R-factor | 0.1919 |
Rwork | 0.191 |
R-free | 0.25200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3I1M 3I1N 3I1O 3I1P |
RMSD bond length | 0.006 |
RMSD bond angle | 1.427 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 3.420 |
High resolution limit [Å] | 3.194 | 7.110 | 3.194 |
Rmerge | 0.072 | 0.036 | 0.435 |
Number of reflections | 708760 | ||
<I/σ(I)> | 12.7 | ||
Completeness [%] | 82.6 | 93 | 69.7 |
Redundancy | 2 | 2.9 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD, pH 6.5, microbatch, temperature 291K |