4V7S

Crystal structure of the E. coli ribosome bound to telithromycin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	210.759, 433.272, 618.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.218 - 3.255
R-factor	0.1943
Rwork	0.193
R-free	0.24500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3I1M 3I1N 3I1O 3I1P
RMSD bond length	0.006
RMSD bond angle	0.923
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	100.000	100.000	3.310
High resolution limit [Å]	3.250	8.820	3.250
Rmerge	0.083	0.043	0.455
Number of reflections	801811
<I/σ(I)>	15.2
Completeness [%]	91.2	97.4	81.1
Redundancy	2.5	4.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD, pH 6.5, microbatch, temperature 291K