Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 236.116, 127.739, 532.668 |
Unit cell angles | 90.00, 102.85, 90.00 |
Refinement procedure
Resolution | 29.994 - 3.005 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | yeast 20S proteasome |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.3.1) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.520 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.103 | 0.314 |
Number of reflections | 416217 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 98.6 | 88.6 |
Redundancy | 5.7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |