Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.230, 448.510, 633.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.248 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.4) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 13.420 | 3.000 |
| Rmerge | 0.307 | 0.014 | 0.014 |
| Number of reflections | 1121164 | 13489 | 42523 |
| <I/σ(I)> | 3.86 | 11.9 | 0.3 |
| Completeness [%] | 95.1 | 96.1 | 49.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K |
