4V7L

The structures of viomycin bound to the 70S ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	210.230, 448.510, 633.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.000
Rwork	0.248
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	PHASER (1.4)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.080
High resolution limit [Å]	3.000	13.420	3.000
Rmerge	0.307	0.014	0.014
Number of reflections	1121164	13489	42523
<I/σ(I)>	3.86	11.9	0.3
Completeness [%]	95.1	96.1	49.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5% Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K