4V7G
Crystal Structure of Lumazine Synthase from Bacillus Anthracis
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.201, 222.237, 473.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.998 - 3.500 |
| R-factor | 0.236 |
| Rwork | 0.235 |
| R-free | 0.28620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rvv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.599 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.290 | 0.860 |
| Number of reflections | 194243 | |
| <I/σ(I)> | 5.2 | 2.2 |
| Completeness [%] | 93.3 | 93.5 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 36% polypropilenglycol P400, 100mM Tris, 20mM DTT, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
