4V7D
Structure of the Ribosome with Elongation Factor G Trapped in the Pre-Translocation State (pre-translocation 70S*tRNA*EF-G structure)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK II |
| Method | Freshly glow-disharged grids were loaded into an FEI Mark II Vitrobot and equilibrated to 95% relative humidity at 22 degrees Celsius. 2 microliters of sample was applied through the side port, blotted for 7 seconds with a positional offset of 2, and plunged into liquid ethane. |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | FEI FALCON I (4k x 4k) |
| Number of Images | 13341 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 7.6 |
| Software | CTFFIND,CNS,UCSF Chimera,EMAN,FREALIGN,IMAGIC,RSAMPLE |
| Symmetry | C1 |
