4V7B
Visualization of two tRNAs trapped in transit during EF-G-mediated translocation
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK I |
| Method | blot for 5-10 seconds before plunging |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | KODAK SO-163 FILM |
| Number of Images | 308 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.8 |
| Software | UCSF Chimera,SPIDER |
| Symmetry | C1 |
