4V74
70S-fMetVal-tRNAVal-tRNAfMet complex in hybrid pre-translocation state (pre5b)
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
Method | Manual blotting for about 2 seconds |
EM Data Collection
Microscope | FEI/PHILIPS CM200FEG |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 160 |
Cs (mm) | 2 |
Detector | GENERIC TVIPS (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 17 |
Software | DireX,Custom,IMAGIC,SPIDER |
Symmetry | C1 |