4V6T

Structure of the bacterial ribosome complexed by tmRNA-SmpB and EF-G during translocation and MLD-loading

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I
Method	blot for 5-10 seconds before plunging

EM Data Collection

Microscope	FEI POLARA 300
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2
Detector	KODAK SO-163 FILM
Number of Images	302

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	8.3
Software	UCSF Chimera,SPIDER
Symmetry	C1