4V6Q
Structural characterization of mRNA-tRNA translocation intermediates (class 5 of the six classes)
This is a non-PDB format compatible entry.
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
Method | blot for 3 seconds |
EM Data Collection
Microscope | FEI TECNAI F30 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.26 |
Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 11.5 |
Software | MDFF,SPIDER,Xmipp |
Symmetry | C1 |