4V6H
Crystal structure of succinate-semialdehyde dehydrogenase from Burkholderia pseudomallei
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Collection date | 2008-04-03 |
| Wavelength(s) | 1.03317 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 185.656, 164.873, 278.901 |
| Unit cell angles | 90.00, 92.01, 90.00 |
Refinement procedure
| Resolution | 49.470 - 2.700 |
| R-factor | 0.238 |
| Rwork | 0.236 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ek1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.104 | 0.692 |
| Number of reflections | 453409 | |
| <I/σ(I)> | 10.3 | 1.6 |
| Completeness [%] | 98.6 | 98.2 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2 mg/mL protein, crystal tracking ID 108914b8, PACT screen B8, 0.1 M MES pH 6.1, 20% PEG 6000, 0.2 M ammonium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
