4V6C
Crystal structure of the E. coli 70S ribosome in an intermediate state of ratcheting
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 211.887, 434.930, 622.921 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.883 - 3.190 |
R-factor | 0.197 |
Rwork | 0.195 |
R-free | 0.25200 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.452 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 200.000 | 200.000 | 3.260 |
High resolution limit [Å] | 3.190 | 8.690 | 3.190 |
Rmerge | 0.171 | 0.045 | |
Number of reflections | 905214 | ||
<I/σ(I)> | 6.925 | ||
Completeness [%] | 96.2 | 98.5 | 93.7 |
Redundancy | 4.7 | 8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 291 | 4% PEG 8000, 4% MPD, 3.8 mM MgCl2, 380 mM NH4Cl, 5.5 mM putrescine, 5 mM spermidine, microbatch, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
10 | 1 | 2 | NH4Cl | ||
11 | 1 | 2 | putrescine | ||
12 | 1 | 2 | spermidine | ||
2 | 1 | 1 | MPD | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | NH4Cl | ||
5 | 1 | 1 | putrescine | ||
6 | 1 | 1 | spermidine | ||
7 | 1 | 2 | PEG 8000 | ||
8 | 1 | 2 | MPD | ||
9 | 1 | 2 | MgCl2 |