4V6A

Structure of EF-P bound to the 70S ribosome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-04
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	209.530, 447.030, 622.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 3.100
Rwork	0.252
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB Entries: 2j00 2j01
RMSD bond length	0.020
RMSD bond angle	1.280
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		3.180
High resolution limit [Å]	3.100	3.100
Rmerge	0.258	0.012
Number of reflections	989777	38341
<I/σ(I)>		0.8
Completeness [%]	92.2	49.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCL, 0.1 M Tris pH 7.6, 2.5 % Peg 20K, 7-12% MPD, 0.5 mM BME, vapor diffusion, temperature 292K