4V67
Crystal structure of a translation termination complex formed with release factor RF2.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.240, 456.780, 618.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.951 - 3.000 |
| R-factor | 0.2806 |
| Rwork | 0.280 |
| R-free | 0.31640 |
| Starting model (for MR) | PDB ENTRIES 3D5A 3D5B 3D5C 3D5D |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS (1.2) |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.951 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 1148216 | |
| <I/σ(I)> | 8.8 | 2.4 |
| Completeness [%] | 97.9 | 89.9 |
| Redundancy | 16.8 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 100 MM TRIS-OAC, 200 MM KSCN, 3.5-4.5 % (W/V) PEG20K, 4-9 % PEG200, DEOXY BIGCHAP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | TRIS-OAC | ||
| 10 | 1 | 2 | DEOXY BIGCHAP | ||
| 2 | 1 | 1 | KSCN | ||
| 3 | 1 | 1 | PEG20K | ||
| 4 | 1 | 1 | PEG200 | ||
| 5 | 1 | 1 | DEOXY BIGCHAP | ||
| 6 | 1 | 2 | TRIS-OAC | ||
| 7 | 1 | 2 | KSCN | ||
| 8 | 1 | 2 | PEG20K | ||
| 9 | 1 | 2 | PEG200 |
