4V60
The structure of rat liver vault at 3.5 angstrom resolution
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-24 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 702.246, 383.796, 598.480 |
| Unit cell angles | 90.00, 124.69, 90.00 |
Refinement procedure
| Resolution | 204.000 - 3.500 |
| Rwork | 0.311 |
| R-free | 0.33040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | cryo-EM map |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.479 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | DM |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 204.000 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.233 | 0.011 |
| Number of reflections | 1531361 | |
| <I/σ(I)> | 5.7 | 1.2 |
| Completeness [%] | 92.4 | 81.2 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 288 | 2.4% PEG 4000, 0.8M sodium chloride, 0.05M lithium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
