Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 283.600, 295.500, 394.300 |
| Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
| Resolution | 49.799 - 3.700 |
| R-factor | 0.2323 |
| Rwork | 0.232 |
| R-free | 0.24690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y26 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.800 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.270 | 0.580 |
| Number of reflections | 682148 | |
| <I/σ(I)> | 8.4 | 2 |
| Completeness [%] | 99.0 | 87.6 |
| Redundancy | 6.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | SITTING DROP (VAPOR DIFFUSION) COMPOSED OF 1:1 MIXTURE OF 1.5 MG/ML GFLV SOLUTION AND OF THE RESERVOIR SOLUTION: 3.75% (M/V) PEG3350, 0.1M NA-HEPES PH 7.5, 0.2% (M/V) AGAROSE GEL. |
