4V5O
CRYSTAL STRUCTURE OF THE EUKARYOTIC 40S RIBOSOMAL SUBUNIT IN COMPLEX WITH INITIATION FACTOR 1.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-08 |
| Detector | DECTRIS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 320.520, 362.210, 412.110 |
| Unit cell angles | 90.00, 109.61, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.930 |
| R-factor | 0.2065 |
| Rwork | 0.206 |
| R-free | 0.24310 |
| Structure solution method | SAD |
| Starting model (for MR) | 2J02 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.338 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.170 |
| High resolution limit [Å] | 3.930 | 3.930 |
| Rmerge | 0.150 | 0.810 |
| Number of reflections | 364651 | |
| <I/σ(I)> | 6.8 | 2.05 |
| Completeness [%] | 93.0 | 61 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 50MM MES-KOH PH6.5, 80MM MGCL2, 200MM KCL, 0.495MM PUTRESCEINE, 4.4-5.4% (W/V) PEG20000 |
