4V5O
CRYSTAL STRUCTURE OF THE EUKARYOTIC 40S RIBOSOMAL SUBUNIT IN COMPLEX WITH INITIATION FACTOR 1.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-03-08 |
Detector | DECTRIS |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 320.520, 362.210, 412.110 |
Unit cell angles | 90.00, 109.61, 90.00 |
Refinement procedure
Resolution | 25.000 - 3.930 |
R-factor | 0.2065 |
Rwork | 0.206 |
R-free | 0.24310 |
Structure solution method | SAD |
Starting model (for MR) | 2J02 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.338 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 4.170 |
High resolution limit [Å] | 3.930 | 3.930 |
Rmerge | 0.150 | 0.810 |
Number of reflections | 364651 | |
<I/σ(I)> | 6.8 | 2.05 |
Completeness [%] | 93.0 | 61 |
Redundancy | 3.4 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 50MM MES-KOH PH6.5, 80MM MGCL2, 200MM KCL, 0.495MM PUTRESCEINE, 4.4-5.4% (W/V) PEG20000 |