4V5I
Structure of the Phage P2 Baseplate in its Activated Conformation with Ca
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 219.521, 219.337, 392.432 |
| Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
| Resolution | 44.563 - 5.464 |
| R-factor | 0.2912 |
| Rwork | 0.291 |
| R-free | 0.29740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2x53 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.600 | 5.800 |
| High resolution limit [Å] | 5.464 | 5.464 |
| Rmerge | 0.070 | 0.460 |
| Number of reflections | 112351 | |
| <I/σ(I)> | 12.3 | 2.8 |
| Completeness [%] | 91.4 | 87.2 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20C, PROTEIN 4 MG/ML. 3.3% PEG 8000 IN 33 MM TRIS PH 8.5, 130 MM CA ACETATE, 66 MM LI SULFATE, AND 66 MM IMIDAZOLE. |
