4V55

Crystal structure of the bacterial ribosome from Escherichia coli in complex with gentamicin and ribosome recycling factor (RRF).

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	93
Detector technology	CCD
Collection date	2006-10-01
Detector	ADSC QUANTUM 315
Wavelength(s)	1.111
Spacegroup name	P 21 21 21
Unit cell lengths	208.540, 378.887, 736.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 4.000
Rwork	0.261
R-free	0.30500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2AVY 2AW4 2AW7 2AWB
RMSD bond length	0.003
RMSD bond angle	0.952
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	139.000	4.400
High resolution limit [Å]	4.000	4.000
Rmerge	0.083	0.287
Number of reflections	382905
<I/σ(I)>	12.1	2.2
Completeness [%]	79.2	32.6
Redundancy	3.2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	batch	7.5	277	MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K
1	batch	7.5	277	MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	MPD
10	1	2	MPD
11	1	2	PEG 8000
12	1	2	MgCl2
13	1	2	NH4Cl
14	1	2	EDTA
2	1	1	PEG 8000
3	1	1	MgCl2
4	1	1	NH4Cl
5	1	1	spermine
6	1	1	spermidine
7	1	1	TRIS
8	1	1	EDTA
9	1	1	H2O