4V4X
Crystal structure of the 70S Thermus thermophilus ribosome showing how the 16S 3'-end mimicks mRNA E and P codons.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-26 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 0.98014 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 509.520, 509.520, 804.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 300.000 - 5.000 |
| R-factor | 0.266 |
| Rwork | 0.263 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.065 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 5.090 |
| High resolution limit [Å] | 5.000 | 5.000 |
| Rmerge | 0.078 | |
| Number of reflections | 223223 | |
| <I/σ(I)> | 9.1 | 2.7 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 7.9 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | MPD | ||
| 4 | 1 | 2 | H2O |
