Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97889 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 518.993, 518.993, 365.139 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 6.200 |
High resolution limit [Å] | 5.900 | 5.900 |
Number of reflections | 149562 | |
<I/σ(I)> | 11.3 | 2.3 |
Completeness [%] | 95.4 | 96.4 |
Redundancy | 7.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.7 | 277 | PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG20K | ||
10 | 1 | 2 | ammonium chloride | ||
2 | 1 | 1 | MES | ||
3 | 1 | 1 | magnesium acetate | ||
4 | 1 | 1 | potassium chloride | ||
5 | 1 | 1 | ammonium chloride | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | PEG20K | ||
8 | 1 | 2 | magnesium acetate | ||
9 | 1 | 2 | potassium chloride |